Minimizing Photobleaching in Fluorescence Microscopy
Minimizing Photobleaching in Fluorescence Microscopy
Oct 24, 2018
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SOLVED:Light-induced degradation of molecules, also called photobleaching, is a serious problem in applications that require very high intensities. A molecule of a fluorescent dye commonly used to label biopolymers can withstand about 10^6 excitations by photons before light-induced reactions destroy its πsystem and the molecule no longer fluoresces. For how long will a single dye molecule fluoresce while being excited by 1.0 mW of 488 nm radiation from a continuous-wave laser? You may assume th
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