Δ crtB RC/YFP–LH1 has a peak at 517 nm corresponding to YFP (indicated by arrow); this peak is shifted to 519 nm at 77 K. (d) Fluorescence excitation spectra of membranes with emission ...

On contrary, recently generated mutants of GFP with yellow-shifted (YFP) and cyan-shifted (CFP) emission spectrum are highly stable and brighter, when compared to BFP.

This emission is observed after excitation at 405 or 458 nm (Supplementary Fig. 3) thus preventing discrimination of CFP and photobleached YFP on the basis of their emission or excitation spectra.

More precisely, as each fluorophore has a unique excitation spectrum and a unique emission spectrum, each peaking at a specific wavelength, they can be distinguished by either their excitation ...