When creating a PCR protocol, users normally concentrate on the template DNA, reaction mix (buffer, dNTPs, taq polymerase), primers, probes, and the thermocycler, which is the main instrument used to ...

Digital PCR, or dPCR, is a molecular biology technique that detects and quantifies nucleic acid targets. By partitioning a sample into many individual reactions, scientists can measure whether a ...

PCR is a technique used to amplify target DNA in a sample. It’s a well-known method that has undergone numerous modifications to enhance its capabilities. This year, it’s turning 40 years old. PCR has ...

Gene-Analysis Studies Advanced by Use of Low-Volume High-Throughput Real-Time PCR This article describes three gene-analysis studies using the Roche LightCycler® 1536 Real-Time PCR System (Figure 1).

PCR amplification can seem straightforward, but in single-cell workflows even small miscalculations can quickly complicate results and downstream analysis. Conventional PCR systems require users to ...

From starting out as a night-drive idea to becoming a global diagnostic gold standard, PCR has irreversibly transformed life sciences in its 40 years of existence. By 1991, scientists had begun ...