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In our studies with COS cells expressing CFP-tagged EGF receptor and YFP-tagged mutant PTP1B (D181A,Q262A), we observed large increases in what we thought was CFP fluorescence when the YFP was ...
3) thus preventing discrimination of CFP and photobleached YFP on the basis of their emission or excitation spectra. During photobleaching FRET experiments, any increase in the CFP channel could ...
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