The total internal reflection which generates the evanescent wave usually occurs at the interface between the sample and the glass coverslip, but the interface can be located deeper in the sample.

The body hairs of the Saharan silver ant cause total internal reflection of light to make the ants almost ten times more reflective, preventing overheating and yielding their silver sheen ...

Total internal reflection microscopy is a light microscopy technique that relies on total internal reflection of illumination light to generate an evanescent wave that extends less than 200 nm ...

The DeltaVision OMX V4 enables Total Internal Reflection Fluorescence Microscopy (TIRF). This technique uses an evanescent wave to selectively excite fluorophores that are close to the coverslip. This ...

Total internal reflection inside a 90 o glass prism The critical angle for the type of glass used to make the prism = 42 o. The ray of light is turned through 180 o.

Cool operator: the Saharan silver ant Cataglyphis bombycina. (Courtesy: P Landmann) A species of desert-dwelling ant is able to tolerate temperatures exceeding 50 °C because its body hairs act like ...

David N. Bowser, Baljit S. Khakh, Two Forms of Single-Vesicle Astrocyte Exocytosis Imaged with Total Internal Reflection Fluorescence Microscopy, Proceedings of the National Academy of Sciences of the ...

Total internal reflection fluorescence (or TIRF) microscopy excites fluorophores in a thin region of the specimen. This way, only fluorescent molecules that are close to the solid (usually a glass ...