NcoI - NEB
WEBNcoI has a High Fidelity version NcoI-HF ® (NEB #R3193). High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity.
NcoI-HF - NEB
WEBHigh Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity.
Compatible Cohesive Ends and Generation of New Restriction Sites | NEB
WEBNew restriction sites can be generated by ligation of DNA fragments with compatible cohesive or blunt ends. These new restriction sites may be generated by: Cleavage followed by fill-in of 5´ overhangs to generate blunt ends. Cleavage with two restriction endonucleases that produce blunt ends.
Restriction Endonucleases Products | NEB
WEBOver 210 restriction enzymes are 100% active in a single buffer – rCutSmart™ Buffer. >180 restriction enzymes are Time-Saver qualified, meaning you can digest DNA in 5-15 minutes, or digest DNA safely overnight. Choose from >265 restriction enzymes, the largest selection commercially available.
NEBcutter V2.0
WEBNEBcutter V2.0. This tool will take a DNA sequence and find the large, non-overlapping open reading frames using the E.coli genetic code and the sites for all Type II and commercially available Type III restriction enzymes that cut the sequence just once.
NotI-HF - NEB
WEBHigh Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity.
NEBuffer Activity/Performance Chart with Restriction Enzymes | NEB
WEBNEB’s restriction enzyme buffer system makes your restriction digests easy and convenient. We are able to offer >210 restriction enzymes that cut in a single buffer, rCutSmart™ . This improves ease-of-use, especially when performing double digests.
NEBcloner
WEBUse NEBcloner to find the right products and protocols for each in your traditional cloning workflow, including double digestion buffers.
NcoI, recombinant | New England Biolabs - neb-online.de
WEBNcoI, recombinant Packaging Unit: 1.000 units. 72,00 -+ Add to Cart
Double Digest Protocol with Standard Restriction Enzymes | NEB
WEBMar 24, 2021 · Digesting a DNA substrate with two restriction enzymes simultaneously (double digestion) is a common timesaving procedure. Over 210 restriction enzymes are 100% active in rCutSmart™ Buffer, making double digestion simple.
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